An immobilized cell is a cell that has been immobilized. Immobilized cells are used as biocatalysts.
Immobilized cells are an alternative to purified and immobilized enzymes and are used when purification of the enzyme is problematic or multiple sequential enzymatic reactions are required. The immobilized cells are most often used in a bioreactor with continuous fluid flow. In some cases, the substances used for immobilization are similar to those used in 3D cell culture. For this, the reaction products must be isolated from the culture medium in the case of live immobilized cells, as opposed to isolation from a buffer in the case of dead immobilized cells. Compared to immobilized enzymes, immobilized cells are often less expensive because there is no protein purification to isolate the enzyme. However, other enzymes are also present in immobilized cells, which can result in more undesirable reaction products.
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